Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012 for this...
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for injury repair.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Extracellular Vesicles From Mesenchymal Stromal Cells Drive Muscle and Neuronal Regeneration Through TNFα Modulation.

Journal of extracellular vesicles·2026
Same author

Colorectal Cancer Hepatic Metastasis Modeling by Advanced 3D Bioprinting Allows Demonstration of Oncolytic Viral Chemotherapeutic Delivery.

Cancers·2025
Same author

Three-dimensional multicellular layer structure: an advanced in vitro model for studying inflammatory bowel diseases.

Scientific reports·2025
Same author

Spotlight on innovation: key insights from the extracellular vesicle cluster meeting at the European Innovation Council.

Cytotherapy·2025
Same author

High Hopes for the Biofabrication of Articular Cartilage-What Lies beyond the Horizon of Tissue Engineering and 3D Bioprinting?

Biomedicines·2024
Same author

Extracellular Vesicles From Mesenchymal Umbilical Cord Cells Exert Protection Against Oxidative Stress and Fibrosis in a Rat Model of Bronchopulmonary Dysplasia.

Stem cells translational medicine·2023

Related Experiment Video

Updated: Jun 2, 2026

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes
10:48

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Published on: April 12, 2015

miRNAs stem cell reprogramming for neuronal induction and differentiation.

Claire Perruisseau-Carrier1, Marcin Jurga, Nico Forraz

  • 1CTI-LYON, Cell Therapy Research Institute, Parc Technologique de Lyon Saint-Priest, Saint-Priest, Lyon, France.

Molecular Neurobiology
|May 5, 2011
PubMed
Summary

MicroRNAs (miRNAs) are key regulators of stem cell differentiation into neurons. Modulating these molecules offers promising avenues for developing new cellular therapies for neurological conditions.

More Related Videos

Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies
12:13

Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies

Published on: July 11, 2019

Lineage-reprogramming of Pericyte-derived Cells of the Adult Human Brain into Induced Neurons
09:36

Lineage-reprogramming of Pericyte-derived Cells of the Adult Human Brain into Induced Neurons

Published on: May 12, 2014

Related Experiment Videos

Last Updated: Jun 2, 2026

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes
10:48

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Published on: April 12, 2015

Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies
12:13

Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies

Published on: July 11, 2019

Lineage-reprogramming of Pericyte-derived Cells of the Adult Human Brain into Induced Neurons
09:36

Lineage-reprogramming of Pericyte-derived Cells of the Adult Human Brain into Induced Neurons

Published on: May 12, 2014

Area of Science:

  • Stem cell biology
  • Neuroscience
  • Molecular biology

Background:

  • Replicating the brain's natural environment is crucial for effective in vitro neuronal differentiation of stem cells.
  • MicroRNAs (miRNAs) have emerged as powerful tools for modulating stem cell lineage commitment and differentiation.
  • Neurotrophin and neuropoietin signaling pathways are central to neuronal development.

Purpose of the Study:

  • To review the role of miRNAs in neuronal differentiation.
  • To highlight the interplay between stem cell signaling and brain-specific pathways.
  • To identify key miRNA candidates for neuronal commitment.

Main Methods:

  • Literature review focusing on miRNA modulation in stem cell differentiation.
  • Analysis of signaling pathways involved in neurogenesis.
  • Identification of miRNA targets and regulatory networks.

Main Results:

  • Transient overexpression or inhibition of specific miRNAs in vitro can direct stem cells towards neuronal lineages.
  • miRNA expression modulation provides a mechanism for post-transcriptional gene regulation in stem cell commitment.
  • Understanding miRNA genetic networks reveals their potential in cellular therapies.

Conclusions:

  • miRNAs are critical regulators of neuronal differentiation from stem cells.
  • Targeting miRNA pathways offers a promising strategy for regenerative medicine and neurological therapies.
  • Further research into miRNA networks will advance the development of human cellular therapies.