Enkephalin-dopamine interactions in the central amygdalar nucleus during gastric stress ulcer formation in rats

Intra-amygdalar (i/am) microinjections of the enkephalin analog, (D-Ala2)-Met-enkephalinamide (DAME, 3, 10 and 30 micrograms) into the central amygdalar nucleus (CEA) produced a dose-related, naltrexone-reversible attenuation of cold restraint (3 h at 4 degrees C)-induced gastric mucosal lesions in rats. Similarly, gastric stress ulcer formation was also inhibited by i/am dopamine (DA, 10 micrograms) - an effect which was reversed by the DA-antagonist, clozapine (5 mg/kg) pretreatment. Further, pretreatment of rats with clozapine or the DA-neurotoxin, 6-hydroxydopamine (6-OHDA, 10 micrograms, i/am) clearly reversed and/or antagonized the gastric cytoprotective effect of DAMEA (30 micrograms). The results indicate interactions between enkephalinergic and DAergic systems at the level of the CEA in the maintenance of gastric mucosal integrity during immobilization stress.