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Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins
09:27

Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins

Published on: January 17, 2014

The host phosphoinositide 5-phosphatase SHIP2 regulates dissemination of vaccinia virus

Shannon McNulty1, Kimberly Powell, Christophe Erneux

  • 1Microbiology and Molecular Genetics Graduate Program, Emory University School of Medicine, Atlanta, Georgia 30322, USA.

Journal of Virology
|May 6, 2011
PubMed

Insights

Poxvirus actin tail formation is regulated by SHIP2, a phosphatase. This protein and viral A34 control virus release, acting as gatekeepers for poxvirus dissemination.

Area of Science:

  • Virology
  • Cell Biology
  • Biochemistry

Background:

  • Enveloped poxviruses utilize actin tails for cell-to-cell spread.
  • Host and viral proteins mediate actin tail formation, but release regulation is less understood.

Purpose of the Study:

  • Investigate the role of phosphoinositide 5-phosphatase SHIP2 in poxvirus actin tail formation and release.
  • Determine the relationship between SHIP2, viral protein A34, and virus dissemination.

Main Methods:

  • Localization studies of SHIP2 in infected cells.
  • Analysis of actin tails and virus release in SHIP2-deficient cells.
  • Examination of virus release and SHIP2 recruitment in cells infected with A34 mutant strains.

Main Results:

  • SHIP2 localizes to actin tails, dependent on tyrosine kinases and N-WASP.
  • Cells lacking SHIP2 exhibit normal actin tails but increased virus release.
  • Viral A34 mutations reduce SHIP2 recruitment and enhance virus release, indicating A34's inhibitory role is mediated by SHIP2.

Conclusions:

  • SHIP2 acts as a negative regulator of poxvirus release.
  • SHIP2 and viral A34 function as gatekeepers to control poxvirus spread based on environmental cues.

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