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Kidney complications in EMC virus-induced diabetes in conventional DBA/2 male mice
K Shichinohe1, M Shimizu, M Ishizaki
1Department of Laboratory Animal Science, Nippon Medical School, Tokyo, Japan.
Abstract:
Conventional DBA/2 male mice of about 9 weeks of age were inoculated by intraperitoneally injecting EMC virus M variant (10(4) TCID50/0.1 ml/animal) which is passaged in mice. The mice which tested positive for glycosuria and hyperglycemia were examined histopathologically 2 or 5 months after inoculation. The kidneys were examined for thickening of Bowman's capsule and the mesangial matrix. These changes were more clearly observable 5 months after inoculation than they were 2 months after inoculation.
Insights
Encephalomyocarditis virus (EMC virus) infection in mice induced kidney changes like thickened Bowman's capsule. These histopathological alterations, indicative of kidney disease, were more pronounced five months post-infection.
Area of Science:
- Virology
- Pathology
- Nephrology
Background:
- Encephalomyocarditis virus (EMC virus) is known to cause various diseases.
- Viral infections can lead to significant organ damage, including kidney pathology.
- DBA/2 mice are a common model for studying disease progression.
Purpose of the Study:
- To investigate the long-term histopathological effects of EMC virus M variant infection on mouse kidneys.
- To assess the development of renal changes following viral inoculation.
- To correlate viral infection with specific kidney structural alterations.
Main Methods:
- DBA/2 male mice were intraperitoneally inoculated with EMC virus M variant.
- Mice testing positive for glycosuria and hyperglycemia were selected for analysis.
- Kidney tissues were examined histopathologically at 2 and 5 months post-inoculation.
- Specific focus on thickening of Bowman's capsule and mesangial matrix.
Main Results:
- Histopathological examination revealed thickening of Bowman's capsule and mesangial matrix in infected mouse kidneys.
- These renal changes were observed in mice exhibiting glycosuria and hyperglycemia.
- The observed kidney pathologies were more evident 5 months after viral inoculation compared to 2 months.
Conclusions:
- EMC virus infection in DBA/2 mice can lead to progressive histopathological changes in the kidneys.
- The development of glycosuria and hyperglycemia may be associated with viral-induced renal damage.
- Longer observation periods (5 months) reveal more pronounced kidney alterations following EMC virus infection.