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Structural and functional relationships between mouse and hamster zona pellucida glycoproteins.

C C Moller1, J D Bleil, R A Kinloch

  • 1Department of Cell and Developmental Biology, Roche Institute of Molecular Biology, Roche Research Center, Nutley, New Jersey 07110.

Developmental Biology
|February 1, 1990
PubMed
Summary

Hamster egg zona pellucida glycoproteins hZP1, hZP2, and hZP3 were identified. hZP3 acts as the sperm receptor, mediating sperm binding and acrosome reaction, similar to mouse ZP3.

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Area of Science:

  • Reproductive Biology
  • Glycobiology
  • Mammalian Reproduction

Background:

  • The zona pellucida (ZP) is a crucial glycoprotein layer surrounding mammalian oocytes.
  • Understanding ZP composition and function is vital for reproductive research and contraception development.
  • Hamster ZP glycoproteins (hZP1, hZP2, hZP3) exhibit heterogeneity and require characterization.

Purpose of the Study:

  • To identify and characterize the specific hamster zona pellucida glycoproteins.
  • To determine the sperm receptor activity of hamster ZP glycoproteins.
  • To investigate functional similarities between hamster and mouse ZP proteins.

Main Methods:

  • Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to analyze glycoprotein heterogeneity.

Related Experiment Videos

  • In vitro competition assays using purified hamster ZP glycoproteins and gametes.
  • Analysis of mRNA expression using a mouse ZP3 cDNA probe.
  • In vitro assays to assess acrosome reaction induction and ZP2-proteinase activity.

    • Main Results:

    • Hamster zona pellucida comprises hZP1, hZP2, and hZP3 glycoproteins.
    • hZP3 functions as the primary sperm receptor, inhibiting sperm-egg binding.
    • Solubilized hamster ZP preparations induce acrosome reactions in both hamster and mouse sperm.
    • hZP2 undergoes proteolysis post-activation, mediated by a ZP2-proteinase similar to the mouse enzyme.

    Conclusions:

    • hZP3 is the functional homolog of mouse ZP3, acting as the hamster sperm receptor.
    • Hamster and mouse ZP glycoproteins share structural and functional similarities, particularly in sperm binding and ZP modification.
    • These findings contribute to understanding interspecies ZP interactions and evolution.