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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Competitive Genomic Screens of Barcoded Yeast Libraries
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Competitive Genomic Screens of Barcoded Yeast Libraries

Published on: August 11, 2011

MassCode liquid arrays as a tool for multiplexed high-throughput genetic profiling.

Gregory S Richmond1, Htet Khine, Tina T Zhou

  • 1Agilent Technologies, Santa Clara, California, United States of America. gregory_richmond@agilent.com

Plos One
|May 6, 2011
PubMed
Summary

A new MassCode PCR method enables high-throughput, sensitive, and cost-effective DNA testing for pathogen identification. This flexible genotyping system offers robust qualitative detection for diagnostics and outbreak monitoring.

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Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
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Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

Area of Science:

  • Molecular Biology
  • Genetics
  • Analytical Chemistry

Background:

  • Multiplexed nucleic acid detection is crucial for pathogen typing, outbreak monitoring, and diagnostics.
  • Existing DNA testing platforms often lack high-throughput, sensitivity, and cost-effectiveness for mid-plexed analysis.

Purpose of the Study:

  • To develop an enhanced, high-throughput, and sensitive DNA testing system for mid-plexed analysis.
  • To demonstrate a flexible genotyping method for robust qualitative differential detection of DNA targets.

Main Methods:

  • Developed an enhanced genotyping method using MassCode technology and MassCode PCR (MC-PCR) with mass-tagged primers.
  • Utilized Lambda exonuclease and MassCode probes for target sequence interrogation in homogeneous solution.
  • Employed a benchtop single quadrupole mass spectrometer for automated detection of cognate tags in MassCode hybrids.

Main Results:

  • Successfully developed a high-throughput mid-plexing analytical system for DNA target detection.
  • Demonstrated a 14plex proof-of-concept assay for subtyping Salmonella enterica serogroups and serovars.
  • Showcased the system's flexibility for customizable test panels.

Conclusions:

  • The MassCode-based system provides a robust, sensitive, and cost-effective solution for mid-plexed DNA analysis.
  • This technology offers advantages over micro- or nanoparticle suspension arrays due to homogeneous solution hybridization.
  • The system is highly adaptable for various multiplexed bioanalysis applications, including diagnostics and pathogen surveillance.