Antibody-dependent cytotoxic activity on human cancer cells expressing UK 114 tumor membrane antigen
A Bartorelli1, B Bussolati, M Millesimo
1UNIV TURIN,DEPT BIOMED SCI & ONCOL,I-10126 TURIN,ITALY. UNIV TURIN,DEPT NEPHROUROL,I-10126 TURIN,ITALY. UNIV TURIN,DEPT BIOL & CLIN SCI,I-10126 TURIN,ITALY. UNIV MILAN,G SISINI INST,I-20138 MILAN,ITALY.
Abstract:
The monoclonal antibody (P-3 mAb) and the rabbit immune sera (RIS) recognizing a 14 kDa perchloric acid soluble protein extracted from goat liver (UK 114), locate this antigen on the cell membrane of several human cancer cell lines. UK 114-positive cells (e.g. HT 29 and KATO VI cells) undergo antibody-dependent cytolysis bl vitro. In nu/nu mice bearing xenografted HT 29 cells, tumor growth was markedly impaired by peri-tumoral injection of anti-UK 114 antibodies. These experiments suggest that human tumors expressing UK 114 over the cell membrane may undergo antibody-mediated cytolysis and growth control.
