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Updated: Jun 2, 2026

A Three-Dimensional Spheroid Model to Investigate the Tumor-Stromal Interaction in Hepatocellular Carcinoma
Published on: September 30, 2021
Fluted serum relating to growth of both hepatocyte and hepatocellular carcinoma cells
Abstract:
This study was undertaken to investigate a humoral substance contributing to both primary hepatocytes and hepatocellular carcinoma (HCC) cells in the primitive form. Peripheral blood from rat, 24 h after partial hepatectomy (PH) or sham-operation, was eluted into 50 fractions using an HPLC, and each fraction was bio-assayed. In vivo relationship between PH and tumor growth was also evaluated. Only one, approximately 100 kDa, of the 50 fractions from PH rat stimulated the growth of both types of cell, and the time-lag between PH and HCC injection also correlated significantly with tumor appearance and growth. Serum in PH rats contains certain humoral stimulator(s) contributing to growth of both hepatocyte and HCC cells.
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