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Aspirin and aspirin-like drugs induce HLA-DR expression in HT29 colon cancer cells

P Arvind1, L Qiao, E Papavassiliou

  • 1CORNELL UNIV,COLL MED,DEPT MED,NEW YORK,NY 10021.

International Journal of Oncology
|May 6, 2011
PubMed

Insights

Nonsteroidal anti-inflammatory drugs (NSAIDs) increase HLA-DR expression in colon cancer cells, potentially enhancing immune surveillance. This suggests NSAIDs may impact colon cancer development by modulating immune responses.

Area of Science:

  • Oncology
  • Immunology
  • Pharmacology

Background:

  • Nonsteroidal anti-inflammatory drugs (NSAIDs) are known to reduce colon cancer incidence.
  • Underexpression of Human Leukocyte Antigen (HLA) is implicated in colon cancer immune evasion.
  • HT29 human colon adenocarcinoma cells lack baseline HLA-DR expression.

Purpose of the Study:

  • To investigate the effect of NSAIDs on HLA-DR expression in colon adenocarcinoma cells.
  • To determine if NSAIDs can restore HLA-DR expression in cells lacking it.

Main Methods:

  • Treatment of HT29 cells with aspirin, indomethacin, and sulindac.
  • Concentration- and time-dependent analysis of HLA-DR expression.
  • Measurement of HLA-DR alpha steady-state mRNA levels and gene transcription rate.

Main Results:

  • Aspirin, indomethacin, and sulindac significantly induced HLA-DR expression in HT29 cells.
  • The induction of HLA-DR was concentration- and time-dependent.
  • Aspirin treatment elevated HLA-DR alpha mRNA levels and gene transcription.

Conclusions:

  • NSAIDs can upregulate HLA-DR expression in colon adenocarcinoma cells.
  • This NSAID-mediated increase in HLA-DR may enhance immune surveillance against colon cancer.
  • The findings suggest a potential in vivo mechanism for NSAIDs in colon cancer prevention.

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