A deuterium-labelling mass spectrometry-tandem diode-array detector screening method for rapid discovery of naturally
Xiaoyu Zhang1, Liping Luo, Zhongjun Ma
1School of Pharmaceutical Sciences, Zhejiang University, Zijingang Campus, Hangzhou, China.
Abstract:
Because electrophiles regulate many signalling pathways in cells, by modifying cysteine residues in proteins, they have a wide range of biological activity. In this study, a deuterium-labelling mass spectrometry-tandem diode-array detector (MS-DAD) screening method was established for rapid discovery of naturally occurring electrophiles. Glutathione (GSH) was used as a probe and incubated with natural product extracts. To distinguish different types of electrophile, incubation was performed in two reaction solvents, H(2)O and D(2)O. Ten types of naturally occurring electrophile were chosen, on the basis of their properties, to undergo the screening assay. By using this screening method, we successfully discovered the bioactive electrophile 4-hydroxyderricin in an ethanol extract of Angelica keiskei. This electrophile had potent NAD(P)H:quinone oxidoreductase 1 (NQO1)-inducing activity at a concentration of 20 μmol L(-1).
More Related Videos
12:11Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
Published on: February 27, 2020
09:00Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1
Published on: April 16, 2018
Related Concept Videos
Tandem Mass Spectrometry
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)
High-Performance Liquid Chromatography: Types of Detectors
