[Subpopulation of CD14+CD16+ monocytes in the children with acute lymphoblastic leukemia treated with filgrastim]
Karol Ratomski1, Marcin Płonowski, Beata Zelazowska-Rutkowska
1Uniwersytet Medyczny w Białymstoku, Zakład Laboratoryjnej Diagnostyki Pediatrycznej.
Unlabelled:
It is well known that patients with leucopenia are susceptible to life-threatening infections due to chemotherapy. The number of neutrophils and monocytes is increasing after administration filgrastim (G-CSF) in patients with the acute lymphoblastic leukemia. CD16 expression was analyzed in order to determine whether monocytes function is also impaired by chemotherapy CD16. The aim of study was to compare the percentage of CD14+CD16+ monocytes in the group of children with the acute lymphoblastic leukemia after administration filgrastim with the group of children with inflammatory state and the group of the healthy children.
Materials And Methods:
The cell surface expression of CD16 was analyzed by flow cytometry. The percentage of CD16 expression and the mean intensity fluorescence rate of CD16 receptor was studied.
Results:
Monocytes showed increased CD16 expression in the group of the children with the acute lymphoblastic leukemia in comparison to the other two groups. There were no differences in the mean fluorescence intensity of the CD16 monocytes between groups.
Conclusions:
The present study demonstrated that G-CSF increased the percentage of monocytes with the CD16 expression, which could be beneficial to the immunological response of the children with the acute lymphoblastic leukemia.
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