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Teeth

Teeth

The formation of teeth, also known as odontogenesis, is a complex process that begins in utero, around the sixth week of embryonic development. There are three stages to this process: the bud stage, the cap stage, and the bell stage.
In the bud stage, the tooth germ (an aggregation of cells) starts to form in the developing jawbone. During the cap stage, the tooth germ differentiates into enamel organ, dental papilla, and dental sac, which will later develop into the tooth's enamel, dentin and...

Cryo-electron Microscopy

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...

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Related Experiment Video

Updated: Jun 2, 2026

Establishing Organoids from Human Tooth as a Powerful Tool Toward Mechanistic Research and Regenerative Therapy

Establishing Organoids from Human Tooth as a Powerful Tool Toward Mechanistic Research and Regenerative Therapy

Published on: April 13, 2022

[Cryopreservation of teeth].

Melanie Zimmerli¹, Andreas Filippi

¹Universitätskliniken für Zahnmedizin der Universität Basel, Klinik für zahnärztliche Chirurgie, -Radiologie, Mund- und Kieferheilkunde.

Schweizer Monatsschrift Fur Zahnmedizin = Revue Mensuelle Suisse D'Odonto-Stomatologie = Rivista Mensile Svizzera Di Odontologia E Stomatologia

|May 7, 2011

Summary

Cryopreservation offers a biological solution for tooth replacement in young patients. Stored healthy teeth can be successfully transplanted, maintaining periodontal ligament cell vitality.

Area of Science:

Dentistry
Regenerative Medicine
Cryobiology

Context:

Dental implants and prosthetics are unsuitable for children and adolescents due to ongoing jaw development.
Tooth loss in this demographic necessitates alternative, biologically compatible replacement strategies.
Cryopreservation provides a method for preserving avulsed or orthodontically removed teeth.

Purpose:

To evaluate cryopreservation as a viable method for storing teeth for future autotransplantation.
To assess the vitality of periodontal ligament cells after cryopreservation and transplantation.
To explore cryopreserved teeth as a biological alternative to conventional tooth replacement in pediatric patients.

Summary:

Healthy teeth removed for orthodontic or traumatic reasons can be cryopreserved using cell culture medium and DMSO.

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Rapid Isolation of Single Cells from Mouse and Human Teeth

Rapid Isolation of Single Cells from Mouse and Human Teeth

Published on: October 28, 2021

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Last Updated: Jun 2, 2026

Establishing Organoids from Human Tooth as a Powerful Tool Toward Mechanistic Research and Regenerative Therapy

Establishing Organoids from Human Tooth as a Powerful Tool Toward Mechanistic Research and Regenerative Therapy

Published on: April 13, 2022

Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth

Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth

Published on: May 17, 2024

Rapid Isolation of Single Cells from Mouse and Human Teeth

Rapid Isolation of Single Cells from Mouse and Human Teeth

Published on: October 28, 2021

Cryopreserved teeth, when used for autotransplantation, demonstrate preserved periodontal ligament cell vitality.

This technique avoids enamel and dentinal cracks, ensuring the structural integrity of the transplanted tooth.

Impact:

Cryopreservation of teeth presents a promising, biological approach for tooth replacement in developing individuals.
This method supports the potential for natural tooth function and integration following transplantation.
Successful cryopreservation and transplantation could significantly improve long-term dental outcomes for children and adolescents.