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Updated: Jun 2, 2026
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Intracellular Refolding Assay
Published on: January 24, 2012
Bioanalysis Young Investigator: Sadagopan Krishnan
1Inorganic Chemistry Laboratory, South Parks Road, Oxford, UK. sadagopan.krishnan@chem.ox.ac.uk
Bioanalysis
|May 7, 2011
Summary
This research introduces novel electrochemiluminescent arrays for toxicity screening and develops biomimetic thin films for cytochrome P450 research. These advancements offer new tools for bioanalytical chemistry and drug metabolism studies.
Area of Science:
- Bioanalytical Chemistry
- Biochemistry
- Materials Science
Background:
- Development of electrochemiluminescent arrays for chemical toxicity screening using cytochrome P450 metalloenzymes.
- Investigation of fundamental properties of human metabolic cytochrome P450s, elucidating the role of iron spin state in enzyme electron transfer rates.
Discussion:
- Creation of thin films that mimic the natural cytochrome P450 redox cycle.
- Implementation of a superparamagnetic labeling scheme for highly sensitive protein immunosensing via surface plasmon resonance (SPR) down to 10 fg/ml.
Key Insights:
- Novel electrochemiluminescent arrays enable efficient chemical toxicity screening.
- Understanding iron spin state's role in P450 electron transfer provides fundamental biochemical insights.
- Biomimetic thin films offer new platforms for studying P450 enzyme mechanisms.
- Advanced SPR techniques achieve unprecedented sensitivity in protein detection.
Outlook:
- Continued research into bioanalytical chemistry applications, including biofuel cells.
- Future goal of establishing a leading research group in bioanalytical chemistry at a major university.
