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A rapid DNA extraction method for PCR amplification from wetland soils
Aims:
We tested a method of rapid DNA extraction from wetland soil samples for use in the polymerase chain reaction.
Methods And Results:
The glass bead/calcium chloride/SDS method obtained in the present study was compared with the calcium chloride/SDS/enzymatic extraction method and the UltraClean™ Soil DNA Isolation Kit. Rapid DNA extraction could be completed within about two hours without purification steps.
Conclusions:
This study succeeded in establishing a fast soil DNA extraction protocol that can be applied to various environmental sources that are rich in humic acid content.
Significance And Impact Of The Study:
The method provides a technology with high-quality DNA extraction from soils for testing the diversity of AOB and AOA.

