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Updated: Jun 2, 2026

Application of Long-term cultured Interferon-γ Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle
Published on: July 11, 2015
Stability of recombinant bovine interferon-γ antiviral activity in the absence of stabilizing additives
Qingyuan Xu1, Tsuyoshi Nomura, Masahiro Ikeda
1Harbin Veterinary Research Institute, 427 Maduan Street, Harbin 150001, China.
Abstract:
The stability of recombinant bovine interferon-γ (rbIFN-γ) produced by a baculovirus expression system was investigated under different storage conditions: freezing-thawing and storage for 30 days at temperatures of -80, 4, 25, and 37°C. Antiviral activity was not significantly decreased by freeze-thawing at least five times. Furthermore, although not statistically different, antiviral activity gradually decreased as temperature increased. These findings suggest that rbIFN-γ possesses high thermal and freeze-thaw stability.
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