Covalent enzyme immobilization by poly(ethylene glycol) diglycidyl ether (PEGDE) for microelectrode biosensor...

Area of Science:

• Biomaterials Science
• Biosensor Technology
• Neuroscience

Background:

• Poly(ethylene glycol) diglycidyl ether (PEGDE) is a common cross-linking agent.
• Its use for direct protein immobilization on biosensors remains underexplored.

Purpose of the Study:

• To investigate PEGDE as a sole immobilizing agent for enzymes on microelectrode biosensors.
• To evaluate the performance and stability of these novel biosensors.

Main Methods:

• Fabrication of microelectrode biosensors using glucose oxidase, d-amino acid oxidase, and glutamate oxidase immobilized with PEGDE.
• In vitro characterization of sensitivity and response time.
• In vivo implantation in rat brains to monitor glucose levels.

Main Results:

• PEGDE-based biosensors demonstrated high sensitivity and rapid response times (seconds).
• Enzymatic activity remained stable for months at 4 °C and days at 37 °C.
• Successful in vivo monitoring of brain glucose fluctuations in rats.

Conclusions:

• PEGDE is a viable, cost-effective, and non-toxic agent for immobilizing enzymes for in vivo biosensor applications.
• This method simplifies biosensor preparation and enhances stability for neurological monitoring.