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Updated: Jul 25, 2026

Electroporation of Functional Bacterial Effectors into Mammalian Cells
Published on: January 19, 2015
Co-transfer of restriction endonucleases and plasmid DNA into mammalian cells by electroporation: effects on stable
1Department of Pediatrics, Faculty of Medicine, Kyoto University, Japan.
Abstract:
We co-transferred cloned HSV thymidine kinase gene and restriction endonucleases, HindIII, XbaI, or XhoI, into thymidine kinase-deficient mouse LtK- cells by electroporation. Stimulation of the transformation efficiency was observed with HindIII or XbaI, while little effect was observed with XhoI. The stimulation was observed for various forms of exogenous DNA (linear or circular plasmid DNA and single-stranded phage DNA) and correlated with the number of strand breaks in the host-cell DNA. These results suggest that the presence of DNA double-strand breaks stimulates the integration of exogenous DNA into host cell genomes.
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