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Standards for Quantitative Metalloproteomic Analysis Using Size Exclusion ICP-MS
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Standards for Quantitative Metalloproteomic Analysis Using Size Exclusion ICP-MS

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Quantitative determination of small selenium species in human serum by HPLC/ICPMS following a protein-removal,

Sabine Kokarnig1, Doris Kuehnelt, Michael Stiboller

  • 1Institute of Chemistry-Analytical Chemistry, Karl-Franzens-University, Graz, Austria.

Analytical and Bioanalytical Chemistry
|May 7, 2011
PubMed

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In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then passed on to...