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Updated: Jun 2, 2026
A Bioluminescent and Fluorescent Orthotopic Syngeneic Murine Model of Androgen-dependent and Castration-resistant Prostate Cancer
Published on: March 6, 2018
[Characterization of prostate cancer cell lines and their epithelial-mesenchymal transition in subcutaneous tumors]
Lin Zhao1, Yong-Guang Jiang, Jie Ma
1Beijing Institute of Heart, Lung and Blood Vessel Diseases, Department of Urology, Beijing Anzhen Hospital Affiliated to Capital Medical University, Beijing 100029, China.
Objective:
To identify the differences in the expression of epithelial or mesenchymal standard proteins between prostate cancer cell lines and tumors, and to investigate the relationship between the process of the prostate cancer cell line forming subcutaneous tumors and epithelial-mesenchymal transition (EMT) by comparing the characteristics of different prostate cell lines forming subcutaneous tumors in SCID mice.
Methods:
We constructed prostate cancer models in male SCID mice by subcutaneous injection of 4 human prostate cancer cell lines DU145, Tsu, PC3 and LNCaP, and compared the characteristics of tumor formation. We used Western blot to detect the expressions of E-cadherin and Vimentin in the cancer cell lines and subcutaneous tumors, observed their differences before and after tumor formation, and explore the relationship between EMT and tumor formation.
Results:
The EMT positive cells DU145 and Tsu showed a higher rate and speed of tumor formation than the EMT negative ones PC3 and LNCaP. The expression of E-cadherin was down-regulated in DU145, up-regulated in Tsu, and absent in PC3 and LNCaP.
Conclusion:
EMT positive cells have a stronger ability of forming tumors than EMT negative cells, and mesenchymal-epithelial transition does exist in subcutaneous tumor formation.
