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Updated: Oct 23, 2026
Reverse Genetics Mediated Recovery of Infectious Murine Norovirus
Published on: June 24, 2012
Mouse hepatitis virus ORF 2a is expressed in the cytosol of infected mouse fibroblasts
P W Zoltick1, J L Leibowitz, E L Oleszak
1Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia 19104-6076.
Abstract:
A 266-bp fragment of cDNA from within gene B, ORF 2a, of MHV-A59 was used to construct a vector encoding a bacterial/viral fusion protein. Antiserum raised against this fusion protein specifically immunoprecipitates a 30K protein from infected 17Cl-1 mouse fibroblasts. The protein is localized primarily in the cytosol and not in the membranes. This is consistent with its predicted sequence and potential role as an RNA binding protein.
Insights
Researchers identified a 30K protein in mouse cells infected with the mouse hepatitis virus (MHV-A59). This protein, likely involved in RNA binding, is located in the cell
Area of Science:
- Virology
- Molecular Biology
- Cell Biology
Background:
- The mouse hepatitis virus (MHV-A59) is a significant pathogen in mouse models.
- Understanding viral protein function is crucial for developing antiviral strategies.
Purpose of the Study:
- To characterize a specific protein encoded by MHV-A59 gene B, ORF 2a.
- To determine the localization and potential function of this viral protein.
Main Methods:
- Construction of a bacterial/viral fusion protein vector using a 266-bp cDNA fragment.
- Generation of antiserum against the fusion protein.
- Immunoprecipitation of infected mouse fibroblasts (17Cl-1 cell line).
Main Results:
- Specific immunoprecipitation of a 30K protein from MHV-A59 infected cells.
- Localization of the 30K protein primarily in the cytosol.
- Exclusion of the protein from cellular membranes.
Conclusions:
- The 30K protein is a cytosolic viral protein encoded by MHV-A59.
- Its localization supports a predicted role as an RNA binding protein.
