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Related Experiment Video

Updated: Jun 2, 2026

Quantitative SERS Detection of Uric Acid via Formation of Precise Plasmonic Nanojunctions within Aggregates of Gold Nanoparticles and Cucurbit[n]uril
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Quantitative SERS Detection of Uric Acid via Formation of Precise Plasmonic Nanojunctions within Aggregates of Gold Nanoparticles and Cucurbit[n]uril

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Compact optical microfluidic uric acid analysis system.

Chia-Pin Chang1, David J Nagel, Manuel T Velasquez

  • 1Department of Electrical and Computer Engineering, The George Washington University, Washington, DC 20052, USA. chiapinc@gwu.edu

Biosensors & Bioelectronics
|May 10, 2011
PubMed
Summary

We developed a compact fluorescence analysis system for rapid uric acid (UA) quantification in saliva, urine, and blood at the point-of-care. This novel system offers accurate UA measurements in just 30 seconds.

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Clinical Diagnostics

Background:

  • Accurate quantification of uric acid (UA) is crucial for diagnosing various health conditions.
  • Current laboratory methods for UA analysis are often time-consuming and require specialized equipment.
  • Point-of-care testing (POCT) offers a promising alternative for rapid diagnostics.

Purpose of the Study:

  • To design, fabricate, and test a novel compact fluorescence analysis system for UA quantification.
  • To enable rapid and accurate UA measurements in clinical samples at the point-of-care.
  • To develop a user-friendly system for analyzing saliva, urine, and blood samples.

Main Methods:

  • A compact fluorescence analysis instrument (7.5 cm × 5 cm × 5 cm) was developed.

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Last Updated: Jun 2, 2026

Quantitative SERS Detection of Uric Acid via Formation of Precise Plasmonic Nanojunctions within Aggregates of Gold Nanoparticles and Cucurbit[n]uril
10:02

Quantitative SERS Detection of Uric Acid via Formation of Precise Plasmonic Nanojunctions within Aggregates of Gold Nanoparticles and Cucurbit[n]uril

Published on: October 3, 2020

Millifluidics for Chemical Synthesis and Time-resolved Mechanistic Studies
12:55

Millifluidics for Chemical Synthesis and Time-resolved Mechanistic Studies

Published on: November 27, 2013

Analyzing Mixing Inhomogeneity in a Microfluidic Device by Microscale Schlieren Technique
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Analyzing Mixing Inhomogeneity in a Microfluidic Device by Microscale Schlieren Technique

Published on: June 12, 2015

  • A disposable thin-film sample holder incorporating a new enzyme immobilization technique was designed.
  • The system utilizes an LED, narrow-band filter, and amplified photodiode for fluorescence detection.
  • Analysis involves simple sample dilution and a 30-second measurement time.

    • Main Results:

    • The system demonstrated a dynamic range of 4-400 μM for uric acid quantification.
    • Calibration curves were established for transparent (saliva, urine) and opaque (blood) samples.
    • Measurements from three types of clinical samples yielded UA concentrations within expected ranges.
    • The analysis time was optimized to 30 seconds per sample.

    Conclusions:

    • The developed compact fluorescence analysis system is effective for point-of-care uric acid quantification.
    • The novel enzyme immobilization technique and instrument design facilitate rapid and accurate measurements.
    • This technology has the potential for development into clinical trial products and widespread POCT applications.