Related Experiment Video
Updated: Jun 2, 2026
14:23
Quantitative Measurement of Invadopodia-mediated Extracellular Matrix Proteolysis in Single and Multicellular Contexts
Published on: August 27, 2012
Invadosome regulation by adhesion signaling
Olivier Destaing1, Marc R Block, Emmanuelle Planus
1INSERM U823, Institut Albert Bonniot, 38706 La Tronche Cedex, Grenoble, France.
Current Opinion in Cell Biology
|May 10, 2011
Summary
Invadosomes, crucial for tissue infiltration, are assembled via integrin signaling. Src and PKC act as key regulators, controlling their formation and function in both normal and cancer cells.
Area of Science:
- Cell Biology
- Biochemistry
- Cancer Research
Background:
- Invadosomes are cellular structures essential for tissue infiltration.
- They consist of an F-actin core and adhesion molecules, functioning as mechanosensory modules.
- Podosomes (physiological) and invadopodia (cancer) are types of invadosomes.
Purpose of the Study:
- To review recent findings on invadosome regulation.
- To highlight the roles of Src and Protein Kinase C (PKC) in invadosome assembly.
- To explain the underlying mechanotransduction and trafficking pathways.
Main Methods:
- Literature review of recent findings on invadosome regulation.
- Analysis of signaling pathways involving integrins, Src, and PKC.
- Examination of mechanotransduction and endo/exocytic trafficking mechanisms.
Main Results:
- Extracellular matrix signals regulate invadosome dynamics through integrin signaling.
- Src acts as an inducer, while PKC amplifies the assembly of integrin-stimulated invadosomes.
- Invadosome function relies on polarized trafficking of proteolytic and enzymatic activities.
Conclusions:
- Invadosome assembly is a complex process orchestrated by extracellular matrix signals.
- Src and PKC play critical, distinct roles in invadosome formation and function.
- Understanding these pathways is crucial for physiological and pathological conditions, including cancer.
Related Concept Videos
01:17Intracellular Signaling Affects Focal Adhesions
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Some...
01:24Adherens Junctions
Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types – adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic
The endothelial cells...
Adherens Junctions are Dynamic
The endothelial cells...
01:35Cancer Cell Migration through Invadopodia
Invadosome is a broad category of cell surface structures with proteolytic activity that degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However, invadopodia can...
01:31Immunoglobulin-like Cell Adhesion Molecules
Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
01:15Activation of Integrins
Integrins bind ligands and transmit information from outside the cell to inside or vice-versa through an "outside-in signaling" or "inside-out signaling."
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding events provide an effective stimulus.
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding events provide an effective stimulus.
01:20Cell Adhesion Molecules - Types and Functions
Cell adhesion molecules (CAMs) are pivotal to multicellularity and the coordinated functioning of tissues and organ systems. They enable physical interactions between cells and provide mechanical strength to tissues. They also function as receptors for signal transmission across the plasma membrane. The CAMs are broadly classified into four families - integrins, cadherins, selectins, and immunoglobulin-like CAMs (IgCAMs).
CAM Families
The Integrin family of proteins is primarily involved in a...
CAM Families
The Integrin family of proteins is primarily involved in a...