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Updated: Jun 2, 2026
09:04
Discovery of New Intracellular Pathogens by Amoebal Coculture and Amoebal Enrichment Approaches
Published on: October 27, 2013
Aquabacterium limnoticum sp. nov., isolated from a freshwater spring
Wen-Ming Chen1, Nian-Tsz Cho1, Shwu-Harn Yang2
1Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, no. 142, Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
Summary
A new bacterial species, Aquabacterium limnoticum, was discovered in a Taiwanese freshwater spring. This facultatively anaerobic bacterium expands the known diversity within the Aquabacterium genus.
Area of Science:
- Microbiology
- Bacteriology
- Environmental Science
Background:
- The genus Aquabacterium comprises aquatic bacteria with diverse metabolic capabilities.
- Understanding bacterial diversity in freshwater ecosystems is crucial for ecological assessments.
Purpose of the Study:
- To characterize a novel bacterial isolate from a freshwater spring using a polyphasic taxonomy approach.
- To determine the phylogenetic and taxonomic position of the novel strain within the Aquabacterium genus.
Main Methods:
- Isolation and cultivation of bacterial strain ABP-4(T) from freshwater.
- Polyphasic taxonomic characterization including 16S rRNA gene sequencing, fatty acid analysis, and DNA-DNA hybridization.
- Determination of optimal growth conditions (temperature, pH, salinity).
Main Results:
- Strain ABP-4(T) is a Gram-negative, facultatively anaerobic rod.
- Phylogenetic analysis based on 16S rRNA gene sequences placed strain ABP-4(T) within the order Burkholderiales, closely related to Aquabacterium species.
- Chemotaxonomic data (fatty acids, isoprenoid quinone, polar lipids) and DNA G+C content (68.6 mol%) were determined.
- DNA-DNA relatedness to known Aquabacterium species was below 70%, supporting its status as a new species.
Conclusions:
- Strain ABP-4(T) represents a novel species within the genus Aquabacterium.
- The proposed name for this new species is Aquabacterium limnoticum sp. nov.
- The type strain is ABP-4(T) (=BCRC 80167(T)=KCTC 23306(T)).
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