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Updated: Jun 2, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
Published on: November 15, 2017
Combining quantitative proteomics data processing workflows for greater sensitivity
Niklaas Colaert1, Christophe Van Huele, Sven Degroeve
1Department of Medical Protein Research, Vlaams Instituut voor Biotechnologie (VIB), Ghent, Belgium.
Abstract:
We here describe a normalization method to combine quantitative proteomics data. By merging the output of two popular quantification software packages, we obtained a 20% increase (on average) in the number of quantified human proteins without suffering from a loss of quality. Our integrative workflow is freely available through our user-friendly, open-source Rover software (http://compomics-rover.googlecode.com/).
