Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

NMR Spectroscopy: Spin–Spin Coupling01:08

NMR Spectroscopy: Spin–Spin Coupling

The spin state of an NMR-active nucleus can have a slight effect on its immediate electronic environment. This effect propagates through the intervening bonds and affects the electronic environments of NMR-active nuclei up to three bonds away; occasionally, even farther. This phenomenon is called spin–spin coupling or J-coupling. Coupling interactions are mutual and result in small changes in the absorption frequencies of both nuclei involved. While nuclei of the same element are involved in...
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)01:15

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are slanted or...
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
NMR Spectrometers: Overview01:20

NMR Spectrometers: Overview

NMR spectrometers consist of a strong magnet, a radiofrequency transmitter, and a detector attached to a computer console for recording spectra of samples containing NMR-active nuclei. In first-generation NMR instruments called continuous-wave spectrometers, the resonance frequencies of the nuclei are determined by frequency-sweep or field-sweep methods. The magnetic field strength is fixed and the rf signal is swept in the former, while the radiofrequency signal is fixed and the magnetic field...
¹³C NMR: ¹H–¹³C Decoupling01:04

¹³C NMR: ¹H–¹³C Decoupling

The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Apical Proximal Tubule Fatty Acid Uptake-Generated Ceramides Cause Endoplasmic Reticulum Stress From Altered Membrane Fluidity.

JCI insight·2026
Same author

Zanidatamab with and without Tislelizumab in HER2-Positive Gastroesophageal Cancer.

The New England journal of medicine·2026
Same author

Asymmetric Rolling-Up Induced Strong Polarization Electric Field for Ultrahigh Areal Electrochemical Capacitance.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Molecular Mechanisms of Traditional Chinese Medicine in Treating Osteoarthritis.

The American journal of Chinese medicine·2026
Same author

Recent Advancements in Chemical Valorization of Legacy and Emerging Fiber-Reinforced Polymer Composites.

ChemSusChem·2026
Same author

Clinicopathological and sonographic characterization of NTRK-fusion papillary thyroid carcinoma based on preoperative molecular testing: a comparative study with BRAF<sup>V600E</sup> PTC.

Frontiers in oncology·2026

Related Experiment Video

Updated: Jun 2, 2026

NMR 15N Relaxation Experiments for the Investigation of Picosecond to Nanoseconds Structural Dynamics of Proteins
09:25

NMR 15N Relaxation Experiments for the Investigation of Picosecond to Nanoseconds Structural Dynamics of Proteins

Published on: November 1, 2024

An Inversion Recovery NMR Kinetics Experiment.

Travis J Williams1, Allan D Kershaw, Vincent Li

  • 1Loker Hydrocarbon Research Institute and Department of Chemistry, University of Southern California, Los Angeles, California 90089-1661, United States.

Journal of Chemical Education
|May 10, 2011
PubMed
Summary

This study introduces a simple laboratory experiment using Nuclear Magnetic Resonance (NMR) magnetization transfer by inversion recovery to determine amide bond rotation kinetics and thermochemistry, offering a versatile method for educational and research purposes.

More Related Videos

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR
10:54

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR

Published on: February 23, 2016

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the &#181;s-ms Timescale
08:09

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale

Published on: April 19, 2021

Related Experiment Videos

Last Updated: Jun 2, 2026

NMR 15N Relaxation Experiments for the Investigation of Picosecond to Nanoseconds Structural Dynamics of Proteins
09:25

NMR 15N Relaxation Experiments for the Investigation of Picosecond to Nanoseconds Structural Dynamics of Proteins

Published on: November 1, 2024

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR
10:54

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR

Published on: February 23, 2016

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the &#181;s-ms Timescale
08:09

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale

Published on: April 19, 2021

Area of Science:

  • Chemical Kinetics
  • Physical Chemistry
  • Spectroscopy

Background:

  • Amide bond rotation is crucial in protein structure and function.
  • Accurate measurement of amide bond rotation kinetics and thermochemistry is essential for understanding molecular behavior.
  • Existing methods may be complex or lack adaptability for diverse laboratory settings.

Purpose of the Study:

  • To present a convenient laboratory experiment for measuring amide bond rotation.
  • To demonstrate the application of Nuclear Magnetic Resonance (NMR) magnetization transfer by inversion recovery for kinetic and thermochemical analysis.
  • To provide a adaptable protocol for both educational and research applications.

Main Methods:

  • Utilized NMR magnetization transfer by inversion recovery.
  • Employed Varian spectrometers with VNMRJ 2.3 software (adaptable to other platforms).
  • Developed specific procedures and sample data sets for data acquisition.

Main Results:

  • Successfully measured the kinetics and thermochemistry of amide bond rotation.
  • The described method is shown to be convenient and adaptable.
  • Provided a template for acquiring inversion recovery data.

Conclusions:

  • Inversion recovery is an effective technique for studying amide bond dynamics.
  • The experiment serves as a valuable laboratory activity for applied NMR courses.
  • The protocol offers a practical template for researchers to obtain inversion recovery data.