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Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
Published on: October 6, 2022
Detection of three-base deletion by exciplex formation with perylene derivatives
Hiromu Kashida1, Nobuyo Kondo, Koji Sekiguchi
1Graduate School of Engineering, Nagoya University, Furocho, Nagoya 464-8603, Japan.
Abstract:
Here, we synthesized fluorescent DNA probes labeled with two perylene derivatives for the detection of a three-base deletion mutant. One such probe discriminated the three-base deletion mutant from the wild-type sequence by exciplex emission, and the deletion mutant was identifiable even by the naked eye.
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