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Updated: Jun 2, 2026
Development of Compendium for Esophageal Squamous Cell Carcinoma
Published on: April 12, 2024
Multiple tumor-suppressor-1 gene and esophageal-carcinoma
1KYUSHU UNIV,MED INST BIOREGULAT,DEPT SURG,BEPPU,OITA 874,JAPAN. SAITAMA CANC CTR,DEPT SURG,INA,SAITAMA,JAPAN. KYOTO UNIV,FAC MED,DEPT SURG 1,KYOTO,JAPAN. UNIV MASSACHUSETTS,MED CTR,DIV DIGEST DIS & NUTR,WORCESTER,MA. KYUSHU UNIV,SCH MED,DEPT SURG 2,FUKUOKA 812,JAPAN.
Abstract:
The multiple tumor suppressor 1 (MTS1) gene is homozygously deleted frequently in cell lines derived from a wide variety of tumors. We investigated the deletion of the MTS1 gene in esophageal cancer cell lines and primary esophageal squamous carcinomas using the polymerase chain reaction. Sixteen and 15 of 23 esophageal cancer cell lines showed homozygous deletion of MTS1 exon 1 and exon 2, respectively, while none of 21 primary esophageal carcinomas showed the deletion. An analysis of MTS1 gene mutations was carried out by direct DNA sequencing in 8 cell lines and 21 primary carcinomas showing no homozygous deletion. In contrast to previous reports of esophageal carcinoma, there were no mutations recognized in the region sequenced. Our study suggests that the inactivation of the MTS 1 gene may play an important role in esophageal carcinoma cell lines but may be less important in primary carcinomas of the human esophagus.
Insights
The multiple tumor suppressor 1 (MTS1) gene is frequently deleted in esophageal cancer cell lines but not in primary tumors. MTS1 gene mutations were also absent in primary esophageal carcinomas, suggesting its inactivation is more relevant in cell lines than in primary esophageal cancers.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- The multiple tumor suppressor 1 (MTS1) gene is frequently deleted in various tumor-derived cell lines.
- Investigating MTS1 alterations is crucial for understanding esophageal cancer development.
Purpose of the Study:
- To examine MTS1 gene deletion and mutation in esophageal cancer cell lines and primary squamous cell carcinomas.
- To determine the role of MTS1 inactivation in esophageal tumorigenesis.
Main Methods:
- Polymerase chain reaction (PCR) was used to detect MTS1 homozygous deletion in exons 1 and 2.
- Direct DNA sequencing analyzed MTS1 gene mutations in cell lines and primary tumors without deletion.
Main Results:
- Homozygous deletion of MTS1 exons 1 and 2 was observed in 16/23 and 15/23 esophageal cancer cell lines, respectively.
- No MTS1 homozygous deletions were found in 21 primary esophageal carcinomas.
- No MTS1 gene mutations were detected in the sequenced regions of either cell lines or primary carcinomas.
Conclusions:
- MTS1 gene inactivation appears significant in esophageal carcinoma cell lines.
- MTS1's role may be less critical in primary human esophageal carcinomas compared to cell line models.
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