Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Study on the pulmonary inflammatory effects induced by inhalation exposure to nanoscale carbon black aerosol in mice].

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]·2015
Same author

Cardioprotective Effects of Total Flavonoids Extracted from Xinjiang Sprig Rosa rugosa against Acute Ischemia/Reperfusion-Induced Myocardial Injury in Isolated Rat Heart.

Cardiovascular toxicology·2015
Same author

Association between TGFBR2 gene polymorphisms and congenital heart defects in Han Chinese population.

Nutricion hospitalaria·2015
Same author

Association between C-reactive protein, incident liver cancer, and chronic liver disease mortality in the Linxian Nutrition Intervention Trials: a nested case-control study.

Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology·2015
Same author

Additional Primary Malignancies in Patients with Gastrointestinal Stromal Tumor (GIST): A Clinicopathologic Study of 260 Patients with Molecular Analysis and Review of the Literature.

Annals of surgical oncology·2015
Same author

Prevalence of potentially traumatic events, depression, alcohol use, and social network supports among Chinese migrants: an epidemiological study in Guangzhou, China.

European journal of psychotraumatology·2014

Related Experiment Video

Updated: Jun 2, 2026

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
14:09

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

Quantitative phase retrieval of complex-valued specimens based on noninterferometric imaging.

Wen Chen1, Xudong Chen

  • 1Department of Electrical and Computer Engineering, National University of Singapore, Singapore. elechenw@nus.edu.sg

Applied Optics
|May 11, 2011
PubMed
Summary
This summary is machine-generated.

We present a novel noninterferometric imaging method for quantitative phase retrieval. This approach simplifies optical setups by avoiding reference waves, making complex specimen analysis more accessible.

More Related Videos

Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture
09:04

Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture

Published on: February 23, 2018

Multimodal Quantitative Phase Imaging with Digital Holographic Microscopy Accurately Assesses Intestinal Inflammation and Epithelial Wound Healing
07:38

Multimodal Quantitative Phase Imaging with Digital Holographic Microscopy Accurately Assesses Intestinal Inflammation and Epithelial Wound Healing

Published on: September 13, 2016

Related Experiment Videos

Last Updated: Jun 2, 2026

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
14:09

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture
09:04

Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture

Published on: February 23, 2018

Multimodal Quantitative Phase Imaging with Digital Holographic Microscopy Accurately Assesses Intestinal Inflammation and Epithelial Wound Healing
07:38

Multimodal Quantitative Phase Imaging with Digital Holographic Microscopy Accurately Assesses Intestinal Inflammation and Epithelial Wound Healing

Published on: September 13, 2016

Area of Science:

  • Optics and Photonics
  • Image Processing
  • Materials Science

Background:

  • Interferometric imaging is used for analyzing crystal and biological tissue structures.
  • Traditional interferometric methods often require complex optical systems, including reference waves and temporal coherence.

Purpose of the Study:

  • To propose a noninterferometric imaging method for quantitative phase retrieval.
  • To overcome the limitations of complex optical setups in interferometric imaging.

Main Methods:

  • Developed a strategy utilizing varying focal lengths within the lens function.
  • Recorded a series of diffraction intensity maps.
  • Employed numerical simulations to validate the method.

Main Results:

  • Demonstrated the feasibility of the proposed noninterferometric quantitative phase retrieval method.
  • Showcased the effectiveness of the strategy using different focal lengths.
  • Numerical simulations confirmed the method's potential for complex-valued specimens.

Conclusions:

  • The proposed noninterferometric imaging method offers a simplified approach to quantitative phase retrieval.
  • This technique is effective for analyzing complex-valued specimens, potentially reducing hardware complexity.