Analyses of linkage to 17q11-q23 in 3 French hereditary nonpolyposis colon-cancer families
D Bernardgallon1, S Gosse, L Essioux
1INSERM,U155,F-75016 PARIS,FRANCE. INST CURIE,F-75005 PARIS,FRANCE. HOP JEAN MINJOZ,F-25030 BESANCON,FRANCE. INST J PAOLI I CALMETTES,F-13273 MARSEILLE 9,FRANCE. CTR LEON BERARD,UNITE ONCOL GENET,F-69373 LYON,FRANCE. LAB IMMUNOL,F-63170 CLERMONT FERRAND,FRANCE. CTR JEAN PERRIN,UNITE PREVENT & DEPISTAGE CANC,ONCOL MOLEC LAB,F-63011 CLERMONT FERRAND,FRANCE.
Abstract:
Hereditary non-polyposis colorectal cancer (HNPCC) is an autosomal dominant disease, accounting for approximately 6% of colorectal cancers. We performed linkage analyses with the aim of proving or excluding the existence of a susceptibility locus on 17q. Three HNPCC families (102 collected members, 25 colorectal cancers, 9 other cancers and 6 colorectal adenomas) were studied with 7 polymorphic DNA markers Mfd15, THRA 1, D17S800, D17S855, Mfd 188, 42D6, 46E6 localized in the 17q11-q23 region. After in vitro enzymatic amplification, the different alleles were separated by classic vertical poly-acrylamide gel electrophoresis or analyzed with the automatic sequencing machine 373A (Applied Biosystems). Results showed that none of the 7 studied markers of the chromosome 17q were linked to the HNPCC disease.
