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Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...

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Related Experiment Video

Updated: Jun 2, 2026

Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

The aberrant p53 protein (review).

M Furihata, H Sonobe, Y Ohtsuki

    International Journal of Oncology
    |May 11, 2011
    PubMed
    Summary
    This summary is machine-generated.

    The p53 tumor suppressor gene plays a critical role in preventing cancer. Detecting p53 abnormalities through immunohistochemistry aids in cancer diagnosis and prognosis.

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    Last Updated: Jun 2, 2026

    Yeast As a Chassis for Developing Functional Assays to Study Human P53
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    Published on: August 4, 2019

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    Purification of Ubiquitinated p53 Proteins from Mammalian Cells
    10:55

    Purification of Ubiquitinated p53 Proteins from Mammalian Cells

    Published on: March 21, 2022

    Area of Science:

    • Molecular oncology
    • Cancer genetics
    • Tumor suppressor genes

    Background:

    • Carcinogenesis involves multistep genetic and epigenetic alterations.
    • Aberrations in oncogenes and tumor-suppressor genes are hallmarks of human cancers.
    • The p53 tumor suppressor gene is frequently implicated in various cancer types.

    Purpose of the Study:

    • To highlight the central role of p53 in human carcinogenesis.
    • To explore the diagnostic, prognostic, and therapeutic implications of p53 abnormalities.
    • To assess the clinical utility of immunohistochemical detection of p53 protein.

    Main Methods:

    • Review of molecular genetics findings related to p53.
    • Discussion of immunohistochemical assays using anti-p53 antibodies.
    • Evaluation of p53 protein accumulation as a biomarker.

    Main Results:

    • Loss of wild-type p53 gene expression is linked to diverse human cancers.
    • Immunohistochemical assessment of p53 protein can provide clinically relevant information.
    • The assay is widely applicable for tumor detection, diagnosis, and prognosis.

    Conclusions:

    • The p53 gene is a key player in cancer development.
    • Immunohistochemistry for p53 protein is a valuable tool in routine cancer investigations.
    • Detection of p53 abnormalities holds significant diagnostic and prognostic potential.