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Related Experiment Videos

Brain lactic acidosis and synaptic function.

W Walz1, D E Harold

  • 1Department of Physiology, College of Medicine, University of Saskatchewan, Saskatoon, Canada.

Canadian Journal of Physiology and Pharmacology
|February 1, 1990
PubMed
Summary

Lactate suppresses synaptic transmission in rat hippocampal slices, but high levels do not cause irreversible damage. However, severe acidosis from lactic acid can irreversibly impair synaptic function.

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Area of Science:

  • Neuroscience
  • Neurophysiology
  • Biochemistry

Background:

  • Lactacidosis, an imbalance of lactic acid, can affect central nervous system function.
  • Understanding the impact of lactacidosis on synaptic transmission is crucial for neurological health.

Purpose of the Study:

  • To investigate the effects of lactacidosis on central synaptic transmission in rat hippocampal slices.
  • To differentiate the impact of lactate concentration versus acidosis on neuronal function.

Main Methods:

  • Electrophysiological recordings in rat hippocampal slices.
  • Measurement of presynaptic fiber volley (PSFV), excitatory postsynaptic potential (EPSP), and extracellular pH.
  • Experimental manipulation using sodium lactate and lactic acid at varying concentrations.

Main Results:

  • Lactate (up to 30 mM) reversibly suppressed EPSP without affecting PSFV.
  • Lactic acid at concentrations causing pH < 6.6 suppressed both PSFV and EPSP irreversibly.
  • Synaptic transmission was more sensitive to lactacidosis than axonal transmission.

Conclusions:

  • Lactate itself has a reversible inhibitory effect on synaptic transmission.
  • Acidosis, not high lactate levels, is responsible for irreversible damage to synaptic transmission.
  • Synaptic transmission is more vulnerable to acidosis than presynaptic axonal transmission.

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