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Related Experiment Video

Updated: Jun 2, 2026

Assays for the Identification of Novel Antivirals against Bluetongue Virus
12:02

Assays for the Identification of Novel Antivirals against Bluetongue Virus

Published on: October 11, 2013

Contamination in bluetongue virus challenge experiments.

Michael Eschbaumer1, Regula Wäckerlin, Giovanni Savini

  • 1Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Südufer 10, 17493 Greifswald-Insel Riems, Germany. michael.eschbaumer@fli.bund.de

Vaccine
|May 12, 2011
PubMed
Summary

Previous bluetongue virus (BTV) exposure to BTV-8 did not prevent reinfection. Unexpectedly, a BTV-15 contaminant dominated cattle infections, while BTV-1 prevailed in sheep, highlighting contamination screening needs.

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Area of Science:

  • Veterinary Virology
  • Infectious Diseases
  • Molecular Diagnostics

Background:

  • Bluetongue virus (BTV) is a significant re-emerging pathogen affecting livestock globally.
  • Understanding cross-protection between different BTV serotypes is crucial for disease control.
  • Experimental infections are vital for studying BTV pathogenesis and immune responses.

Purpose of the Study:

  • To investigate the outcome of sequential BTV infections with different serotypes in cattle and sheep.
  • To evaluate the impact of prior BTV-8 exposure on subsequent infection with BTV-1.
  • To assess the influence of a BTV-15 contaminant on experimental BTV-1 infections.

Main Methods:

  • Inoculation of cattle and sheep with BTV-1, including animals with prior BTV-8 experimental infection.

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Last Updated: Jun 2, 2026

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  • Serotype-specific real-time RT-PCR analysis of inoculum and blood samples.
  • Design of type-specific RT-PCR primer sets for BTV serotypes and Toggenburg Orbivirus.

    • Main Results:

    • Prior BTV-8 exposure did not confer protection against subsequent BTV-1 infection.
    • BTV-15 contaminant unexpectedly dominated BTV-1 infection in cattle previously exposed to BTV-8.
    • BTV-1 prevailed over the BTV-15 contaminant in both naive and BTV-8-exposed sheep.

    Conclusions:

    • Sequential BTV infections can lead to complex outcomes influenced by serotype and prior exposure.
    • Contamination of virus preparations can significantly alter experimental results, as seen with BTV-15 in cattle.
    • Robust contamination screening methods, such as developed RT-PCR assays, are essential for reliable virological studies.