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Updated: Jun 2, 2026

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
Published on: September 25, 2018
Accurate and highly sensitive screening of DNA-binding molecules with primer-dimer templates using a real-time PCR
Akira Inoue1, Tsunehiro Kumeda, Hiroshi Nakamura
1Faculty of Pharmaceutical Sciences, Tokyo University of Science, Chiba, Japan.
Abstract:
We have developed a strategy to detect the DNA-binding affinities of chemical compounds with a real-time PCR analyzer. In this system, the DNA-binding affinities of chemical compounds are efficiency detected by PCR with high sensitivity. To obtain accurate information about the DNA-binding affinities of chemical compounds, primer-dimers prepared in advance are used as templates. The denature temperature is settled to around the melting temperature of the templates.
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