Related Experiment Video
Updated: Jun 2, 2026

Optimized Griess Reaction for UV-Vis and Naked-eye Determination of Anti-malarial Primaquine
Published on: October 11, 2019
Rapid determination of quinoline and 2-hydroxyquinoline in quinoline biodegradation process by tri-wavelength UV/Vis
Xiuqiong Deng1, Xinsheng Chai, Chaohai Wei
1College of Environmental Science and Engineering, South China University of Technology, Guangzhou, PR China.
Abstract:
For the present study, a tri-wavelength UV/Vis spectrophotometric method for rapid determination of quinoline (Q) and 2-hydroxyquinoline (HQ) during Q biodegradation was developed. Based on the spectral measurements at 289 nm (the isosbestic point of Q and HQ), 326 and 380 nm, the spectral interference of extracellular polymeric substances (EPS) in the process samples could be minimized, and the amounts of Q and HQ could be simultaneously quantified. Our results indicated that the relative standard deviations in the repeatability tests were 2.7 and 1.7% for Q and HQ, respectively. The method validation was conducted by comparing the data obtained using the present method with those generated from high performance liquid chromatography (HPLC). The same set of samples from Q biodegradation process was used. The relative differences between the two methods were within 10%. In conclusion, the present method is simple, rapid, and suitable for the investigation in Q biodegradation processes.
Related Concept Videos
Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview
UV–Vis Spectrometers
Spectrophotometry: Introduction
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
UV–Vis Spectrum
The UV–Vis spectrum of a molecule is the plot of its absorbance versus wavelength. The plot is drawn by taking molar absorptivity (ε) or log ε on the y-axis (ordinate)...
Oxidation of Phenols to Quinones
o-hydroxy phenols are oxidized to o-quinones and p-hydroxy phenols to p-quinones. Such redox reactions involve the transfer of two electrons and two protons. The reversible redox property is crucial in...

