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Updated: Jun 2, 2026

Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR
Published on: June 11, 2016
16S rRNA gene microarray analysis of microbial communities in ethanol-stimulated subsurface sediment
Santosh R Mohanty1, Bharati Kollah, Eoin L Brodie
1Department of Geoscience, University of Wisconsin, Madison, 1215 W. Dayton St., WI 53706, USA.
Abstract:
A high-density 16S rRNA gene microarray was used to analyze microbial communities in a slurry of ethanol-amended, uranium-contaminated subsurface sediment. Of specific interest was the extent to which the microarray could detect temporal patterns in the relative abundance of major metabolic groups (nitrate-reducing, metal-reducing, sulfate-reducing, and methanogenic taxa) that were stimulated by ethanol addition. The results show that the microarray, when used in conjunction with geochemical data and knowledge of the physiological properties of relevant taxa, provided accurate assessment of the response of key functional groups to biostimulation.

