Related Experiment Video
Updated: Jun 2, 2026

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing
Published on: March 19, 2018
Global sequencing: a review of current molecular data and new methods available to assess microbial diversity
1Université de Nice et CNRS UMR 6543, Laboratoire de Biologie Virtuelle, Cente de Biochimie, Parc Valrose, Faculté des Sciences, Nice, F 06108 France.
Abstract:
Roughly estimating the diversity of life has for a long time been seen as a challenge. Sequences obtained from the environment by direct amplification are now seen as the sole mean to provide information for at least 99% of the prokaryotes in natural communities. Recent massive parallel sequencing technologies now allow to sequence nearly every sequence resulting from such amplification. Are we ready to deal with such a deluge of data? Using three recent publications (16S rRNA genes amplification in soil and seawater followed by massive parallel sequencing) as case examples, this review analyses the state of the field.
Related Concept Videos
Modern Molecular Taxonomy
Applications of Molecular Taxonomy
Methods to Assess Microbial Communities
Evolutionary Relationships through Genome Comparisons
Next-generation Sequencing
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
RNA-seq
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

