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Related Experiment Video

Updated: Jun 2, 2026

Using a Pan-Viral Microarray Assay (Virochip) to Screen Clinical Samples for Viral Pathogens
13:45

Using a Pan-Viral Microarray Assay (Virochip) to Screen Clinical Samples for Viral Pathogens

Published on: April 27, 2011

Using a pan-viral microarray assay (Virochip) to screen clinical samples for viral pathogens.

Eunice C Chen1, Steve A Miller, Joseph L DeRisi

  • 1Department of Laboratory Medicine, University of California, San Francisco, USA.

Journal of Visualized Experiments : Jove
|May 12, 2011
PubMed
Summary

The Virochip, a pan-viral microarray, effectively detects known and novel viruses, including SARS coronavirus and H1N1 influenza, aiding in diagnosing infectious diseases and unexplained critical illnesses.

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Area of Science:

  • Virology
  • Infectious Diseases
  • Molecular Diagnostics

Background:

  • Diagnosing viral infections is challenging due to viral diversity and emerging pathogens.
  • Traditional methods struggle to detect novel or highly variable viruses like SARS coronavirus and H1N1 influenza.
  • A pan-viral microarray offers a solution for broad-spectrum viral detection.

Purpose of the Study:

  • To demonstrate the Virochip assay processing from sample to result.
  • To highlight the Virochip's capability in detecting a wide range of viral pathogens.
  • To showcase the Virochip's utility in identifying novel viruses and understanding respiratory infections.

Main Methods:

  • Nucleic acid extraction from samples.
  • Random primer PCR amplification and fluorescent dye incorporation.

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Last Updated: Jun 2, 2026

Using a Pan-Viral Microarray Assay (Virochip) to Screen Clinical Samples for Viral Pathogens
13:45

Using a Pan-Viral Microarray Assay (Virochip) to Screen Clinical Samples for Viral Pathogens

Published on: April 27, 2011

High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses
11:34

High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses

Published on: May 5, 2014

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR
11:00

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

Published on: November 28, 2016

  • Microarray hybridization, scanning, and data analysis on the Agilent platform.

    • Main Results:

    • The Virochip identified the full spectrum of viruses in respiratory infections, including critical illness cases.
    • It demonstrated sensitivity equal to or greater than conventional methods.
    • The Virochip has successfully identified novel viruses such as SARS coronavirus, XMRV, and avian bornavirus.

    Conclusions:

    • The Virochip is a validated platform for detecting known and novel viral pathogens.
    • It provides a comprehensive diagnostic tool for infectious diseases, especially respiratory infections.
    • The assay offers a rapid (~24-hour turnaround) and sensitive approach to viral diagnostics.