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Updated: Apr 29, 2026

Transcriptome Analysis of Single Cells

Transcriptome Analysis of Single Cells

Published on: April 25, 2011

Single-cell assays.

Declan Ryan¹, Kangning Ren, Hongkai Wu

¹Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, People's Republic of China.

Biomicrofluidics

|May 12, 2011

Summary

This summary is machine-generated.

Microfluidics enables single-cell assays by analyzing individual cell content. This review covers recent methods and future impacts of 3D cell culture on single-cell analysis.

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Last Updated: Apr 29, 2026

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Viability Assays for Cells in Culture

Viability Assays for Cells in Culture

Published on: January 20, 2014

Area of Science:

Biotechnology
Cell Biology
Microfluidics

Background:

Single-cell assays are crucial for understanding cellular heterogeneity and function.
Quantifying individual mammalian cell content is essential for successful assay development.
Recent advancements in microfluidics offer powerful tools for single-cell analysis.

Purpose of the Study:

To review the applications of microfluidics in single-cell assay development.
To identify key criteria for successful single-cell assays.
To discuss the impact of emerging technologies like 3D cell culture on the field.

Main Methods:

Comprehensive literature review of microfluidic and microfabrication methods for single-cell studies.
Analysis of recent publications (past 24 months) focusing on individual cell assays.
Synthesis of findings to outline critical factors for assay success.

Main Results:

Microfluidics provides essential capabilities for quantifying individual cell content.
Key characteristics for successful single-cell assays have been identified.
Recent literature highlights innovative microfluidic approaches for cell analysis.

Conclusions:

Microfluidics is a transformative technology for single-cell assays.
Future directions include integrating 3D cell culture and chemically defined cells.
Continued innovation in microfluidic platforms will advance single-cell biology.