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Regulatory feedback loop of two phz gene clusters through 5'-untranslated regions in Pseudomonas sp. M18

Yaqian Li1, Xilin Du, Zhi John Lu

  • 1State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.

Plos One
|May 12, 2011
PubMed
Abstract

Insights

Phenazine-1-carboxylic acid (PCA) produced by two gene clusters in Pseudomonas sp. M18 auto-regulates its own expression and activates another cluster. This complex bacterial signaling involves 5'-untranslated regions (UTRs) and the GacA pathway.

Area of Science:

  • Microbiology
  • Bacterial Genetics
  • Molecular Biology

Background:

  • Pseudomonas bacteria produce important phenazine compounds.
  • Two homologous phenazine (phz) gene clusters, phzA1-G1 and phzA2-G2, exist in Pseudomonas sp. M18.
  • The regulatory correlation between these two phz gene clusters is largely unknown.

Purpose of the Study:

  • Investigate the correlation between the expression of the two phz gene clusters in Pseudomonas sp. M18.
  • Elucidate the regulatory mechanisms governing phenazine biosynthesis.
  • Understand the role of phenazine-1-carboxylic acid (PCA) in gene regulation.

Main Methods:

  • Construction of two chromosomal insertion-inactivated mutants for each phz gene cluster.
  • Analysis of gene expression and regulation at transcriptional and post-transcriptional levels.
  • Investigation of the role of the GacA signal transduction pathway.

Main Results:

  • PCA molecules from the phzA2-G2 cluster auto-regulate and activate the phzA1-G1 cluster in a feedback loop.
  • Post-transcriptional expression of the phzA1-G1 transcript is primarily inhibited by its 5 acronym{'-untranslated region (UTR).
  • The phzA2-G2 cluster shows lower transcription but efficient translation, negatively regulated by the GacA pathway at the post-transcriptional level.

Conclusions:

  • PCA acts as a key mediator, produced in varying amounts by both phz gene clusters.
  • A unique regulatory mechanism involving 5 acronym{'-UTRs coordinates complex bacterial signaling.
  • This intricate regulation allows Pseudomonas sp. M18 to manage phenazine production effectively.

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