Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

Computed Tomography

Computed Tomography

Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

CYLD-mediated lysine63 deubiquitination regulates synaptic transmission and autophagy to mitigate age-related sequelae.

Nature communications·2026

Same author

The nuclear shredder behind PARPi resistance.

Nature cell biology·2026

Same author

Pvr and Pvf2 Are Essential for Valve Cell Differentiation in the Larval Drosophila Heart.

Genesis (New York, N.Y. : 2000)·2026

Same author

Quantifying the sensitivity limit of ICG imaging in the presence of tissue autofluorescence.

Journal of biomedical optics·2026

Same author

Endosomal maturation is controlled by the trimeric Bulli-Mon1-Ccz1 Rab7 GEF complex and the Rab5 GTPase-activating protein GAPsec.

Journal of cell science·2026

Same author

Newfoundland Mutation <i>TMEM43</i>-p.S358L Causes Impaired Cardiac Energy Metabolism and Mitochondrial Function Through Altered Protein Interaction.

Circulation. Genomic and precision medicine·2026

Search research articles

Related Experiment Video

Updated: Jun 2, 2026

Thinned-skull Cortical Window Technique for In Vivo Optical Coherence Tomography Imaging

Thinned-skull Cortical Window Technique for In Vivo Optical Coherence Tomography Imaging

Published on: November 19, 2012

Microscopic optical projection tomography in vivo.

Matthias Rieckher¹, Udo Jochen Birk, Heiko Meyer

¹Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, Crete, Greece.

|May 12, 2011

Summary

We developed a novel optical projection tomography system for fast 3D imaging of live microscopic organisms. This advanced microscopy technique provides high-resolution, asymmetric resolution-free imaging for biological research.

More Related Videos

In Vivo Imaging of Cx3cr1gfp/gfp Reporter Mice with Spectral-domain Optical Coherence Tomography and Scanning Laser Ophthalmoscopy

In Vivo Imaging of Cx3cr1^gfp/gfp Reporter Mice with Spectral-domain Optical Coherence Tomography and Scanning Laser Ophthalmoscopy

Published on: November 11, 2017

Mesoscopic Fluorescence Tomography for In-vivo Imaging of Developing Drosophila

Mesoscopic Fluorescence Tomography for In-vivo Imaging of Developing Drosophila

Published on: August 20, 2009

Related Experiment Videos

Last Updated: Jun 2, 2026

Thinned-skull Cortical Window Technique for In Vivo Optical Coherence Tomography Imaging

Thinned-skull Cortical Window Technique for In Vivo Optical Coherence Tomography Imaging

Published on: November 19, 2012

In Vivo Imaging of Cx3cr1gfp/gfp Reporter Mice with Spectral-domain Optical Coherence Tomography and Scanning Laser Ophthalmoscopy

In Vivo Imaging of Cx3cr1^gfp/gfp Reporter Mice with Spectral-domain Optical Coherence Tomography and Scanning Laser Ophthalmoscopy

Published on: November 11, 2017

Mesoscopic Fluorescence Tomography for In-vivo Imaging of Developing Drosophila

Mesoscopic Fluorescence Tomography for In-vivo Imaging of Developing Drosophila

Published on: August 20, 2009

Area of Science:

Biomedical Engineering
Microscopy
Developmental Biology

Background:

Conventional confocal microscopy exhibits asymmetric resolution in xy and z dimensions due to optical sectioning.
Imaging dynamic biological processes in live organisms requires high-resolution techniques with improved spatial accuracy.

Purpose of the Study:

To present a versatile optical projection tomography system for rapid, high-resolution 3D imaging of microscopic specimens in vivo.
To overcome the resolution limitations of conventional microscopy for dynamic biological studies.

Main Methods:

Development of a novel optical projection tomography setup.
Utilizing both fluorescence and absorption contrast for imaging.
Application to live, transparent invertebrate model organisms like C. elegans.

Main Results:

The system achieves robust, high-resolution 3D imaging without asymmetric resolution.
Enabled detailed monitoring of spatio-temporal gene expression and anatomical changes with single-cell resolution.
Demonstrated adaptability for various small model organisms.

Conclusions:

The optical projection tomography system offers significant advantages for studying dynamic developmental processes and aging in vivo.
Provides a powerful tool for advancing biological research requiring precise 3D imaging of live specimens.