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A new electrochemical method for the production of stable ascorbate free radicals
1Department of Science, Middle-East Technical University, Ankara, Turkey.
Journal of Biochemical and Biophysical Methods
|January 1, 1990
Abstract:
A new method for the production of ascorbate free radicals is established. The radical is produced from ascorbate in deionized water by applying constant potential electrolysis under a nitrogen atmosphere. Prior to electrolysis, a cyclic voltammogram (CV) of the ascorbic acid was obtained. Electrolysis potentials were selected as the oxidation peak potential of the ascorbic acid obtained by CV. The detection of the radical was done by electron spin resonance (esr) and uv spectroscopies.