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Preparation of nucleate and anucleate fragments of hamster and mouse eggs by centrifugation
1Department of Anatomy and Reproductive Biology, University of Hawaii School of Medicine, Honolulu 96822.
The Journal of Experimental Zoology
|February 1, 1990
Abstract:
Nucleate and anucleate fragments of hamster and mouse eggs can be prepared by centrifuging zona-free eggs in a Percoll gradient. These fragments are fertilizable and can be used for various studies of nucleus-ooplasm interactions.