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Centrifugation

Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...
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In vitro fertilization (IVF) is a form of assisted reproductive technology where an egg is fertilized with sperm in a controlled laboratory environment before transferring the resulting embryo into the uterus. This process is designed to help individuals and couples experiencing difficulties conceiving.
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Sperm Collection of Differential Quality Using Density Gradient Centrifugation
03:28

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Published on: November 29, 2018

Temperature controlled centrifugation improves sperm retrieval.

D R Franken1, R van Wyk, C Stoumann

  • 1Department of Obstetrics and Gynaecology, University of Stellenbosch, Tygerberg, South Africa. drf@sun.ac.za

Andrologia
|May 13, 2011
PubMed
Summary

Maintaining optimal temperature during sperm preparation significantly improves the retrieval of motile sperm. Temperature-controlled sperm preparation may benefit men with reduced sperm motility.

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Area of Science:

  • Reproductive Biology
  • Andrology
  • Assisted Reproductive Technology

Background:

  • Sperm retrieval techniques are crucial for assisted reproductive programs.
  • Success is typically measured by the quantity of motile sperm obtained.
  • Sperm preparation involves multiple steps, including centrifugation and swim-up procedures.

Purpose of the Study:

  • To investigate the impact of temperature during sperm preparation on sperm retrieval outcomes.
  • To compare the effectiveness of temperature-controlled versus room temperature conditions.
  • To assess the influence of temperature on both sperm concentration and motility.

Main Methods:

  • Thirty semen samples from normozoospermic donors were divided into two groups: 34°C incubation and room temperature (25°C).
  • Sperm preparation involved a double wash swim-up technique using two centrifuges, one with temperature control (34°C) and one without.
  • Sperm concentration and motility were analyzed using a computer-assisted sperm analyzer.

Main Results:

  • Temperature-controlled preparation at 34°C yielded significantly higher mean sperm concentrations compared to room temperature (P < 0.05).
  • The mean percentage of motile sperm retrieved at 34°C was significantly higher than at room temperature (P = 0.02).
  • Centrifuge temperature and incubation temperature demonstrated a significant influence on the percentage of retrieved motile sperm.

Conclusions:

  • Temperature control during sperm preparation positively impacts the number and motility of retrieved sperm.
  • Utilizing temperature-controlled sperm preparation techniques may offer clinical advantages for individuals with impaired sperm motility.
  • Optimizing sperm preparation temperature is a key factor in maximizing the success of assisted reproductive procedures.