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Peroxidase activity in peritoneal macrophages of patients with terminal renal failure treated by intermittent
W Sułowicz1, R Pawlicki, Z Hanicki
1Department of Nephrology, Medical Academy, Kraków, Poland.
Abstract:
Peroxidase activity was assessed cytochemically in macrophages from peritoneal dialysate of 50 patients with terminal renal failure treated by intermittent peritoneal dialysis. In 22 patients cells were harvested for the second time after development of peritonitis as the complication of the treatment. The obtained results were compared with those of the control group consisting of 30 patients with normal renal function. Patients treated with peritoneal dialysis showed both in complication-free period and during the course of peritonitis significantly higher peroxidase activity in macrophages as revealed by the semiquantitative score test. In the course of peritonitis, peroxidase activity in macrophages was significantly lower than in complication-free period. Electron-microscopic cytochemistry demonstrated that in macrophages obtained from both control and dialyzed patients the peroxidase activity was located exclusively in cytoplasmic granules, suggesting a similarity of the studied cells and exudative macrophages.
Insights
Patients undergoing peritoneal dialysis (PD) exhibit elevated macrophage peroxidase activity. This activity decreases during peritonitis, a common PD complication, but remains higher than in healthy individuals.
Area of Science:
- Immunology
- Nephrology
- Cell Biology
Background:
- Peritoneal dialysis (PD) is a treatment for end-stage renal failure.
- Macrophages play a role in the inflammatory response during PD and its complications.
- Peroxidase activity in macrophages is an indicator of cellular activity.
Purpose of the Study:
- To assess peroxidase activity in macrophages of patients undergoing PD.
- To compare peroxidase activity in patients with and without peritonitis.
- To investigate the cellular localization of peroxidase in these macrophages.
Main Methods:
- Cytochemical assessment of peroxidase activity in macrophages from peritoneal dialysate.
- Semiquantitative score test for peroxidase activity.
- Electron-microscopic cytochemistry for subcellular localization.
Main Results:
- Macrophages from PD patients showed significantly higher peroxidase activity compared to controls.
- Peroxidase activity was significantly lower during peritonitis compared to the complication-free period in PD patients.
- Electron microscopy confirmed peroxidase activity localized to cytoplasmic granules in all groups.
Conclusions:
- PD treatment is associated with enhanced macrophage peroxidase activity.
- Peritonitis leads to a decrease in macrophage peroxidase activity during PD.
- The studied macrophages share characteristics with exudative macrophages.