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Related Concept Videos

Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...

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Related Experiment Video

Updated: Jun 2, 2026

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method
07:58

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method

Published on: September 19, 2018

HiTRACE: high-throughput robust analysis for capillary electrophoresis.

Sungroh Yoon1, Jinkyu Kim, Justine Hum

  • 1School of Electrical Engineering, Korea University, Seoul 136-713, Republic of Korea. sryoon@korea.ac.kr

Bioinformatics (Oxford, England)
|May 13, 2011
PubMed
Summary

We developed HiTRACE, a computational method to automate capillary electrophoresis (CE) analysis for nucleic acids. This tool significantly speeds up the analysis of RNA and DNA structure, enabling high-throughput studies.

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Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
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Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Published on: October 24, 2018

Related Experiment Videos

Last Updated: Jun 2, 2026

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method
07:58

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method

Published on: September 19, 2018

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
10:05

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Published on: October 24, 2018

Area of Science:

  • Biochemistry
  • Computational Biology
  • Genomics

Background:

  • Capillary electrophoresis (CE) is crucial for high-throughput genome analysis and nucleic acid structural inference.
  • Current CE analysis tools for RNA and DNA structure are slow and lack automation, hindering large-scale studies.

Purpose of the Study:

  • To develop an automated computational method for large-scale nucleic acid CE analysis.
  • To overcome the limitations of existing tools in terms of speed and automation for CE data.

Main Methods:

  • Developed HiTRACE (high-throughput robust analysis for capillary electrophoresis), a computational method utilizing dynamic programming algorithms.
  • Applied HiTRACE to analyze 13 datasets across 4 RNAs, 3 chemical modification strategies, and up to 480 variants.

Main Results:

  • HiTRACE automates key tasks, including profile alignment, significantly improving efficiency.
  • The method demonstrates superior alignment and fitting quality compared to prior tools.
  • Analysis time reduced from hours of manual intervention to minutes, even for datasets with 87,360 bands.

Conclusions:

  • HiTRACE resolves a critical bottleneck in analyzing nucleic acid structure using CE.
  • The method enables efficient and accurate quantitative analysis for experiments with tens of thousands of bands.
  • Facilitates a new generation of high-throughput studies in RNA and DNA structure, thermodynamics, and kinetics.