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qPCR Is a Sensitive and Rapid Method for Detection of Cytomegaloviral DNA in Formalin-fixed, Paraffin-embedded Biopsy Tissue
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Cytomegalovirus DNA quantification using an automated platform for nucleic acid extraction and real-time PCR assay

Michael Forman1, Andy Wilson, Alexandra Valsamakis

  • 1The Johns Hopkins Medical Institutions, Division of Microbiology, Baltimore, Maryland, USA. mformaa@jhmi.edu

Journal of Clinical Microbiology
|May 13, 2011
PubMed

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Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...