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The B-A transition in superhelical DNA.

Krylov DYu1, V L Makarov, V I Ivanov

  • 1Engelhardt Institute of Molecular Biology, USSR Academy of Sciences, Moscow.

Nucleic Acids Research
|February 25, 1990
PubMed
Summary

Researchers studied DNA

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Area of Science:

  • Molecular Biology
  • Biophysics

Background:

  • DNA exists in various forms, including B-DNA and A-DNA.
  • Trifluoroethanol can induce a transition between these DNA forms.
  • DNA superhelicity influences DNA structure and function.

Purpose of the Study:

  • To investigate the relaxation of DNA superhelical stress during the B-DNA to A-DNA transition.
  • To quantify changes in DNA winding angle during this transition in solution.

Main Methods:

  • Studying DNA orientation changes in a flow gradient.
  • Utilizing DNA with varying superhelical densities.
  • Measuring DNA winding angles in solution.

Main Results:

  • A decrease in DNA winding angle of 1.5 degrees per base pair was observed during the B-A transition.
  • The calculated winding angle for A-DNA in solution is 32.6 degrees, consistent with X-ray data.
  • An increase in winding of approximately 5 degrees per junction occurs at each B/A junction.

Conclusions:

  • The B-DNA to A-DNA transition in solution leads to a reduction in DNA superhelicity.
  • The observed winding angle changes support existing models of DNA conformational transitions.

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