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Characterizing the variation in pH measurements with apheresis platelets
Gary Moroff1, Shalini Seetharaman, James Kurtz
1American Red Cross Biomedical Services, Holland Laboratory, Rockville, Maryland 20855, USA.
Transfusion
|May 14, 2011
Summary
Accurate pH measurement is crucial for platelet (PLT) component quality. Two pH meters demonstrated precise and comparable results, while a blood gas analyzer showed greater variability in PLT pH readings.
Area of Science:
- Biomedical Science
- Transfusion Medicine
- Analytical Chemistry
Background:
- Platelet (PLT) component quality is assessed by pH, which is affected by storage and shipping.
- Accurate pH monitoring is vital for maintaining PLT properties.
Purpose of the Study:
- To evaluate the precision and comparability of pH measurements using two distinct pH meters and a blood gas analyzer.
- To understand variations in pH readings under different measurement conditions.
Main Methods:
- Apheresis PLT units were stored with or without agitation.
- pH was measured using two pH meters at room temperature (20-24°C) and a blood gas analyzer at 37°C (converted to 22°C).
Main Results:
- pH meters showed high precision with a coefficient of variation (%CV) of 0.43% or less.
- The difference in pH values between the two pH meters was minimal (≤0.10 units).
- Greater differences were observed when comparing pH meter readings to the blood gas analyzer results.
Conclusions:
- Two pH meters provide precise and comparable pH measurements for platelet components.
- Blood gas analyzer measurements showed greater variability compared to pH meters.
