Related Experiment Video
Updated: Jun 2, 2026
10:17
Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers
Published on: October 5, 2017
Polymorphic microsatellite markers isolated from the neptune whelk Neptunea arthritica
N Azuma1, R M Miranda, S Goshima
1Graduate School of Fisheries Sciences, Hokkaido University, 3-1-1 Minato-cho, Hakodate-shi 041-8611, Japan.
Molecular Ecology Resources
|May 14, 2011
Summary
Eight new microsatellite DNA markers were identified for the neptune whelk (Neptunea arthritica). These genetic markers show variation useful for studying populations and family relationships in this important fishery species.
Area of Science:
- Marine Biology
- Genetics
- Fisheries Science
Background:
- The neptune whelk (Neptunea arthritica) is a significant fishery resource in northern Japan.
- Understanding the genetic diversity and population structure of this species is crucial for effective fisheries management.
Purpose of the Study:
- To isolate and characterize polymorphic microsatellite DNA loci in Neptunea arthritica.
- To assess the utility of these microsatellite markers for population and kinship analyses.
Main Methods:
- Isolation of eight polymorphic microsatellite DNA loci from Neptunea arthritica.
- Analysis of allele number and heterozygosity (observed and expected) at each locus.
Main Results:
- A total of eight polymorphic microsatellite loci were successfully isolated.
- The number of alleles per locus ranged from two to six.
- Observed heterozygosities ranged from 0.192 to 0.807, and expected heterozygosities ranged from 0.233 to 0.738.
Conclusions:
- The identified microsatellite loci exhibit significant genetic variation within Neptunea arthritica populations.
- These markers are suitable for future population genetics and kinship analyses, aiding in the conservation and management of this valuable fishery resource.
