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Updated: Jun 2, 2026

Tick Microbiome Characterization by Next-Generation 16S rRNA Amplicon Sequencing
Published on: August 25, 2018
Development and characterization of 12 polymorphic microsatellite loci in the American dog tick (Dermacentor
Guha Dharmarajan1, Jennifer A Fike, James C Beasley
1Department of Forestry and Natural Resources, Purdue University, Pfendler Building, West Lafayette, IN 47907, USA.
Abstract:
Twelve polymorphic microsatellite loci were developed for the American dog tick (Dermacentor variabilis), an important vector of infectious diseases in humans and animals. Four multiplexed panels comprising the loci were developed and 45 ticks collected from two raccoons (Procyon lotor) were genotyped. The number of alleles per locus ranged from nine to 30, and single locus heterozygosities ranged from 0.18 to 0.93. Data generated using these markers will further our understanding of factors affecting gene flow in D. variabilis, thus helping to elucidate the transmission dynamics of diseases associated with this vector.
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